Fig. 1: Schematic, point spread function, and the linearity characterization of the 3D-DyFI system.
From: Three-dimensional random-access confocal microscopy with 3D remote focusing system
a Schematic of 3D-DyFI. OL: Objective Lens, M: Mirror, F: fluorescence filter, DM: Dichroic Mirror, PH: Pinhole, PCL: Plano-Concave Lens, APD: Avalanche Photodiode. The x-y scanning images (up panel) and x-z scanning images (low panel) of 50 nm green fluorescent microsphere with (b) piezoelectric stage scanning and (c) 3D-DyFI. The color indicates fluorescence intensity. Scale bar: 500 nm. The laser focus positions as a function of applied voltage in x (d), y (e), and z (f) axes. Measured data are represented by blue circles, while the fitting results are shown with green lines. n = 3.
