Fig. 1: Schematic of EPROI. | npj Imaging

Fig. 1: Schematic of EPROI.

From: Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging

Fig. 1

a Ball-and-stick model of oxygen sensitive spin probe, OX071. Carbon atoms are depicted in grey, hydrogen in white, deuterium in green, oxygen in red, and sulfur in gold. b Inversion recovery electron spin echo pulse sequence. c single exponential recovery fit of the signal as a function of delay, T, that is used to calculate R1 (= 1/T1). d radial k-space acquisition scheme shown in 2D with static gradients that are used for image acquisition. e R1 vs pO2 calibration curves for the materials used in the current project. The slopes and intercepts are: 109.664 torr/Ms−1, 0.181 Ms−1; 123.3941 torr/Ms−1, 0.1537 Ms−1; 113.484 torr/Ms−1, 0.130 Ms−1 for PBS, DMEM cell culture medium, and VitroGel, respectively.

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