Fig. 1: Stimulated Raman histology (SRH) applied to organoids.
From: In vivo organoid growth monitoring by stimulated Raman histology
a A matrigel droplet is mounted between 2 coverslips and placed onto the SRH microscope (b) for virtual histology imaging (c). d Phase contrast image of cancerous organoids looking like a normal rectum organoid (scale bar = 75 µm), e SRH images of the same normal-like organoid acquired at different depths: z step between each image is 5 µm, scale bar = 150 µm, f Phase contrast image of a cancerous organoid (from human rectum), scale bar = 75 µm, g SRH images of the same cancerous organoid acquired at different depths: z step between each image is 5 µm, scale bar = 150 µm, h 3D-reconstruction of the normal-like organoid from SRH images presented in (e), i 3D-reconstruction of the cancerous organoid cut halfway through from SRH images presented in (g), (j) HE image of a normal-like rectum organoid from the same patient, scale bar = 100 µm, k HE image of a cancerous rectum organoid from the same patient, Scale bar = 100 µm.
