Fig. 3: FAP receptor trafficking assessment with live-cell time lapse microscopy and flow cytometry. | npj Imaging

Fig. 3: FAP receptor trafficking assessment with live-cell time lapse microscopy and flow cytometry.

From: Increased imaging ligand hydrophilicity and improved pharmacokinetic properties provides enhanced in vivo targeting of fibroblast activation protein

Fig. 3

A Live-cell time-lapse of HEK293-hFAP cells labeled with continuous exposure of FAPI-AF647. Surface saturation occurs quickly, followed by appearance of punctate red spots, consistent with receptor trafficking. Scale bar in (A) is representative for all images. B In vitro internalization assays with overexpressing and endogenous cells recapitulate the microscopy results. C Plot of internal fraction of surface saturation vs the fractional surface integral. Here, the slope is the 1st-order net internalization rate constant.

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