Fig. 4: In vivo MRI comparison of the monomeric and tetrameric probes in a murine model of vascular remodelling.
From: Non-invasive in vivo imaging of changes in Collagen III turnover in myocardial fibrosis
a Experimental design. b Schematic of the infrarenal aorta undergoing surgery and magnetic resonance angiography (MRA) segment. c Both monomeric probes show enhancement up to 60 min post- injection. d [Gd-DOTAMA]4-CBP1 and [Gd-DOTAMA]4-CBP2 show stronger enhancement at 60 min compared with corresponding monomeric probes e The scrambled tetrameric Scc-CBP1 probe shows negligible enhancement but the Scc-CBP2 probe shows enhancement suggesting unspecific binding of the [Gd-DOTAMA]4-CBP2 probe. f Signal enhancement observed by MRI co-localised with COL3 fibres (green) as seen by histology. L aortic lumen, HFD high-fat diet. Figure 4a and schematic in 4b were created with BioRender.com.
