Fig. 2: Spectral unmixing and dimensionality reduction of hyperspectral EDX.
From: Automated analysis of ultrastructure through large-scale hyperspectral electron microscopy
a EM image corresponding to a single EDX acquisition of a 100 nm thick pancreas section. Representative pixels of insulin (red), heterochromatin (green), glucagon (green), a membranous structure (cyan), intracellular space (magenta) and membranes (yellow) are annotated. b Abundance maps of the HSI using average spectra from the annotated regions in (a) as endmembers. Here, low and high abundances are respectively mapped from white to black and the colour outline corresponds to the annotated feature in (a). c PaCMAP embedding of the HSI (inset) and labelled according to the annotated pixels in (a). d Relative Euclidean distance within classes as a function of frame accumulation. e Ratio of between the relative Euclidean distance within and amongst classes as a function of frame accumulation. Scale is 2 µm.
