Fig. 3: Extraction and identification of endmembers in large-scale EDX.
From: Automated analysis of ultrastructure through large-scale hyperspectral electron microscopy
a Subsampled (20%) PaCMAP embedding of a large-scale EDX acquisition. Endmembers were identified based on their abundance and separation from the bulk (inset). Labelling of the data points reflects the colour of the most abundant endmember found after linear unmixing. b PaCMAP embedding coloured according to the highest abundance value in each pixel. c Large-scale EM (High-angle annular dark-field; HAADF) image of a partial islet of Langerhans (left) and spatial representation of the unmixing result with the colour representing the endmember with the highest abundance and the intensity scaled accordingly (right). d Heatmap reflecting the per channel Z-score for each extracted endmember. Labels are based on the biological features annotated in (d).
