Fig. 2: Region-based reconstruction of quantitative phase image and deconvolution of fluorescence image.

a The bright-field image stack is divided into small regions, and the quantitative phase image of each region is obtained by region-based refocusing and TIE-based phase retrieval. b, c Refocusing and deconvolution of central (b) and corner (c) regions of the fluorescence images of the centromeres labeled against CENP-A. Sub-cellular structure of centromeres is clearly visible. d The improved fluorescence image after region-based refocusing and deconvolution.