Fig. 5: Hyperplex fluorescence images of the inflamed tissue.

The multiplexed fluorescence images were obtained through 4 cycles of immunofluorescence staining and imaging with 16 protein markers. a Representative multi-color fluorescence image of colonic tissue showing active inflammation in a patient with ulcerative colitis. b–e Enlarged multiplexed fluorescence views of the boxed regions in (a), each imaged after one of four cyclic staining-elution cycles.