Fig. 3: DSP identifies significant disease dependent protein expression changes irrespective of laminar cortical and subcortical layers of the brain.

A Heatmap of differentially regulated proteins when comparing all regions of interest. Linear mixed model regression analysis was used to identify significantly upregulated proteins disease groups are indicated as any value above zero but less than p < 0.05. Significantly (p < 0.05) downregulated proteins are indicated by any value below zero. B–DIndividual protein markers of interest independent of subregion specificity (all ROIs merged). Generalized linear regression comparing Log2 normalized gene expression via quantification of oligonucleotide barcode reads (counts) between disease groups was considered significant when p < 0.05. All counts were normalized to ROI area and internal controls.