Figure 3

The Ang II receptor AT1 regulates H7N9-induced lung injury.

(A) The wet-to-dry ratio of the lungs of WT mice treated with control or AT1 inhibitor (losartan, 15 mg/kg) 30 min before Hb01/H7N9 virus infection (n = 8). (B) H&E staining and infiltrating cell counts (n = 200 fields) in the lung tissue of Hb01/H7N9 influenza virus-infected B6 mice treated with PBS control or AT1 inhibitor (losartan, 15 mg/kg) at day 5 post-infection. (C) Detection of Hb01/H7N9 virus NP RNA in WT mice treated with PBS control or AT1 inhibitor (losartan, 15 mg/kg) 30 min before Hb01/H7N9 virus infection (n = 8) at day 5 post-infection. NP mRNA expression was quantified using real-time PCR and was normalized to GAPDH (n = 10). (D) Lung viral titers in WT mice treated with PBS control or AT1 inhibitor (losartan, 15 mg/kg) before Hb01/H7N9 virus infection (n = 8) at day 5 post-infection. **p < 0.01(two-tailed t-test).