Figure 2

Biological function of exosome loaded miRNA-155 mimic.

Hepatocytes and Kupffer cells were isolated from miR-155 KO mice as described in the methods. Exosomes loaded with either scrambled mimic or miR-155 mimic were added to cells for 6 h. Cells were washed 3 times with PBS to remove free-floating exosomes. Hepatocytes were lysed with Qiazole and miR-155 levels were evaluated (A) Kupffer cells were treated or not with LPS (100 ng/ul) for 6 h and cell-free supernatant was collected. The levels of miR-155 (B) MIP2 (C) and MCP1 (mRNA) were checked using real-time qPCR and levels were normalized to untreated cells. MCP1 protein levels were measured from cell-free supernatant (E) miR-155 levels were normalized to hepatocytes or Kupffer cells isolated from wild type mice. * indicates p<0.05 scrambled loaded exosome treated cells and # compared to scrambled loaded exosome +LPS treated cells. SnoRNA202 (A,B) or 18S (C,D) was used to normalize the technical variations between the samples. ns: non significant. Statistical analysis was performed using non-parametric Mann-Whitney test.