Figure 1

The expression of sirt6 in normal and OA knee articular cartilage.
(A) Immunofluorescence analysis of human articular cartilage showed the sirt6 fluorescence intensities were significantly decreased in OA patients relative to normal. (B) The western blotting analysis revealed that the protein level of sirt6 was significantly decreased in the chondrocytes of OA patients. (C) The quantitative RT-PCR showed the expression of sirt6 was significantly lower in the chondrocytes of OA patients than in normal human. 1 & 2 indicated specimens from two people. (D) The thickness and integrity of the articular cartilage were decreased in OA cartilage compared with normal cartilage on safranin O stains. (E) The immunofluorescence stains of the mice cartilage revealed that the sirt6 fluorescence intensities were significantly decreased in aged mice relative to young mice.