Figure 4
From: PSIP1/LEDGF: a new gene likely involved in sensorineural progressive hearing loss
mRNA analysis of PSIP1 gene.
(a) Chromatograms of PSIP1 mRNA show a wild type sequence in the II:2 individual and the presence of the mutated allele in the III:1 and II:1 patients. (b) Quantification of PSIP1 mRNA by real-time PCR in peripheral blood cells. Relative expression ratios represent the amount of mRNA in the PSIP1 +/− patients (II:1;III:1) divided by that in the wild type individual (II:2). No differences were present. Assays were performed in triplicate; standard deviations are shown. (c) Electrophoresis followed by measurement of fluorescence intensity shows no differences in the amount of mutated and wild-type allele in patients and controls. Patients II:1 and III:1 present two peaks compared to the control (II:2) although the area under the peaks is the same.
