Figure 4: Gene-silencing potency and cytotoxicity in cultured cells.
From: Inter-molecular β-sheet structure facilitates lung-targeting siRNA delivery
(A) Gene-silencing activities in cultured HEK293 cells. PC, effective siRNA targeting fusion luciferase gene; naked siRNA, luciferase-target siRNA without any delivery carrier; NC, negative control siRNA with an irrelevant target sequence with regard to the fusion luciferase gene; Lipo, transfection mediated by Lipofectamine 2000. (B) Relative cell viability after exposure to different siRNA/peptide formulations. CTL, untreated cells; naked siRNA, gene-specific siRNA without any carrier. Lipo, cells treated with Lipofectamine 2000. Concentration of siRNA is 33 nM. Only statistically significant associations are indicated. P-values are designated by asterisks (**P < 0.01; ***P < 0.001).
