Figure 2: Comparative analysis of serum proteome alterations in NSVM and SVM patients.

(A) Trends of a few selected differentially abundant proteins in NSVM and SVM identified in 2D-DIGE. Data are represented as standardized log abundance of spot intensity (One representative spot is shown for the proteins with multiple spots in 2D-DIGE gels). (B) Volcano plots showing P values (−log10) versus protein ratio of (log2). Red, up-regulated; Green, down-regulated; and Blue, not significantly changed (adjusted p-value > 0.05) proteins. A few selected differentially abundant proteins are labeled. (C) Representative MS/MS spectrum of a few selected differentially abundant proteins. Inset showing the iTRAQ reporter ion intensities for representative peptides in HC, NSVM and SVM. (D) Venn diagram showing the unique and common differentially abundant proteins (p-value ≤ 0.05) in NSVM and SVM identified in iTRAQ analysis by ESI-Q-TOF. (E) Venn diagram showing the unique and overlapping proteins identified in Q-Exactive and ESI-Q-TOF.