Figure 3: Decreased targeting activities of truncated sgRNAs compared to full-length sgRNAs in iPSCs and iMSCs.
From: Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency
Efficiencies of GFP knockout in iPSCs (A) and iMSCs (B) after transduction of Cas9 and sgRNAs bearing full-length (20nt) or shortened sgRNAs (17nt) that target GFP sites 42, 101, 261 and 379. Lengths and sequences of the sgGFPs are shown. Data shown are mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. (C) A summary of GFP knockout efficiencies by Cas9 and 17nt vs. 20nt sgGFPs that target 4 sites of the GFP reporter gene (sites 42, 101, 261 and 379) in 293T cells, iPSCs and iMSCs. Data shown are mean ± SEM (n = 4 pairs of sgGFPs). ns, not significant; ***P < 0.001. (D) Efficiencies of CD73 knockout in iMSCs after transduction of Cas9 and sgRNAs bearing full-length (20nt) or shortened sgRNAs (17nt) that target the CD73 gene sites a and b. Lengths and sequences of the sgCD73s are shown. Data shown are mean ± SEM (n = 3). *P < 0.05.
