Figure 4: Distinct indel profiles of gene disruptions mediated by 20nt vs. 17nt sgGFP.
From: Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency
(A) Distribution of deletion and insertion mutations induced by Cas9 and 20nt vs. 17nt sgRNAs targeting four sites of the GFP reporter gene (sites 42, 101, 261 and 379). Detailed information on indels is summarized in Fig. S1. (B) Distribution of indel length. The indels were fractionated into 3 groups: 1bp, 2–8 bp, >9 bp. (C) Comparison of average indel length induced by Cas9 and 20nt vs. 17nt sgGFPs. (D) Comparison of in-frame and frameshift GFP mutations induced by 20nt vs. 17nt sgGFPs. Data shown are mean ± SEM (n = 24). *P < 0.05.
