Figure 1

Timeline of experiments for four groups of rats.
Cold-induced hypertension (CIH) rats were randomly divided into EA, sham-EA or untreated hypertension groups. They were treated with repetitive EA or sham-EA at the ST36-37 acupoints, or restraint only, for 30 min twice weekly for another five weeks following staying in the cold room for six weeks. Rats kept at room temperature were restrained for 30 min twice weekly for eleven weeks. Blood pressures (BPs) in all groups of rats were evaluated weekly using tail-cuff. Twenty-four or 72 hrs after five weeks of treatment, rats were decapitated or microinjected to respectively examine the preproenkephalin mRNA levels and role of δ-opioid receptors in the rVLM.