Figure 5
Anatomical locations of microinjection sites in the rat.
Left panel: An original slide of the medulla oblongata (+1.44 mm from the obex) shows blue-dyed track of a microdialysis probe insertion used for injections. Ventral aspect of blue dye represents site of microinjection in the rVLM, as indicated by arrow. Middle and right panels: Composite maps displaying histologically verified sites of microinjections in the rVLM of rats. The middle panel displays a magnified area in the right panel, indicated by the box. Brain section shows composite of planes of brain stem rostral to the obex (Paxinos and Watson’s atlas). Symbols represent microinjection of ICI 174,864 (★), DADLE (◾), naloxone (▴) and injections outside the rVLM (⚪). All injections were bilateral, although for ease of display, sites are superimposed on the left. rVLM, rostral ventrolateral medulla; Py, pyramidal tract; Sp5, spinal trigeminal nucleus; NA, nucleus ambiguus; 4V, 4th ventricle.
