Figure 4

Suppression of autophagy by 3-MA attenuates 5i induced cell viability inhibition.
(a) Co-treatment of 3-MA with 5i in H460 and A549 cells and immunofluorescence assays for detecting LC3 I and LC3 II. (b) Co-treatment of 3-MA with 5i in H460 and A549 cells and western blotting assays for detecting LC3 I and LC3 II expression. (c) Co-treatment of 3-MA with 5i in H460 and A549 cells and MTT assays for detecting viable cells. (d) Co-treatment of 3-MA with 5i in H460 and A549 cells and G1 cell cycle distribution. Data are represented as mean ± SD and p value was calculated with t-test.