Figure 6 | Scientific Reports

Figure 6

From: Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response

Figure 6

HQT attenuates epithelial cell apoptosis and intestinal permeability in DSS-induced acute colitis.

Mice were administered regular water (control) or 3.5% DSS for 7 days followed by treatment with HQT for 7 days. (a) TUNEL assay for apoptosis (magnification, x200). (b) The percent of TUNEL-positive cells. (c) Intestinal permeability assessed by measuring serum FITC-Dextran levels 4 hours after administration. (d) Immunohistochemistry analysis of caspase-3 in colon sections (magnification, ×200). (e) Graphical representation of Caspase-3 positive cells in the mid-colon. (f) Western blotting analyses and densitometric quantitation of colonic mucosal levels of bax and bcl-2 proteins. The gels were run under the same experimental conditions. Results are expressed as mean ± SD of three independent experiments; n = 8 mice per group. *p < 0.05, **p < 0.001 vs. the control group; △p < 0.05, △△p < 0.001 vs. DSS-treated mice. TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labelling; FITC, fluorescein isothiocyanate.

Back to article page