Figure 3: Identification of miR-26b as a target of circRNA_000203. | Scientific Reports

Figure 3: Identification of miR-26b as a target of circRNA_000203.

From: CircRNA_000203 enhances the expression of fibrosis-associated genes by derepressing targets of miR-26b-5p, Col1a2 and CTGF, in cardiac fibroblasts

Figure 3

(A) Predicted binding sites of miR-26b in circRNA_000203. (B) The pulled down circRNA_000203 by miR-26b or miR-26b-mut by RT-qPCR assay. (C) Predicted miR-26b seed matches to the sequences in the 3′UTR of CTGF and Col1a2 mRNA. The seed sequence of miR-26b is UCAAGUA, and the complementary nucleotide sequences are shown in red words. (D) Expressions of miR-26b and circRNA_000203 were determined by RT-qPCR assay. Verification of CTGF (E) and Col1a2 (F) as target genes of miR-26b by the dual luciferase reporter assay. (G) MRNA and protein expression of Col1a2 and CTGF in mouse cardiac fibroblasts with overexpression of miR-26b. Data are shown as mean ± sem, *p < 0.05, **p < 0.01, ***p < 0.001. N = 3.

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