Abstract
THE LIM homeodomain (LIM-HD) proteins, which contain two tandem LIM domains followed by a homeodomain, are critical transcriptional regulators of embryonic development1–5. The LIM ___domain is a conserved cysteine-rich zinc-binding motif found in LIM-HD and LMO (rhombotin or Ttg) proteins, cytoskeletal components, LIM kinases and other proteins1. LIM domains are protein-protein interaction motifs1, can inhibit binding of LIM-HD proteins to DNA6,7 and can negatively regulate LIM-HD protein function8. How LIM domains exert these regulatory effects is not known. We have now isolated a new LIM-___domain-binding factor, Ldbl, on the basis of its ability to interact with the LIM-HD protein Lhxl (Liml) 9. High-affinity binding by Ldbl requires paired LIM domains and is restricted to the related subgroup of LIM domains found in LIM-HD and LMO proteins. The highly conserved Xenopus Ldb protein XLdbl, interacts with Xlim-1, the Xenopus orthologue of Lhxl. When injected into Xenopus embryos, XLdbl (or Ldbl) can synergize with Xlim-1 in the formation of partial secondary axes and in activation of the genes encoding goosecoid (gsc), chordin, NCAM and XCG7, demonstrating a functional as well as a physical interaction between the two proteins.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
199,00 € per year
only 3,90 € per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Dawid, I. B., Toyama, R. & Taira, M. C. R. Acad. Sci. III 318, 295–306 (1995).
Sanchez-Garcia, I. & Rabbitts, T. H. Trends Genet. 10, 315–320 (1994).
Shawlot, W. & Behringer, R. R. Nature 374, 425–430 (1995).
Pfaff, S. L., Mendelsohn, M., Stewart, C. L., Edlund, T. & Jessell, T. M. Cell 84, 309–320 (1996).
Sheng, H. Z. et al. Science 272, 1004–1007 (1996).
Xue, D., Tu, Y. & Chalfie, M. Science 261, 1324–1328 (1993).
Sanchez-Garcia, I., Osada, H., Forster, A. & Rabbitts, T. H. EMBO J. 12, 4243–4250 (1993).
Taira, M., Otani, H., Saint-Jeannet, J.-P. & Dawid, I. B. Nature 372, 677–679 (1994).
Fujii, T. et al. Dev. Dyn. 199, 73–83 (1994).
Taira, M., Jamrich, M., Good, P. J. & Dawid, I. B. Genes Dev. 6, 356–366 (1992).
Taira, M., Evrard, J. L., Steinmetz, A. & Dawid, I. B. Trends Genet. 11, 431–432 (1995).
Nunoue, K., Ohashi, K., Okano, I. & Mizuno, K. Oncogene 11, 701–710 (1995).
Arber, S., Halder, G. & Caroni, P. Cell 79, 221–231 (1994).
Sadler, I., Crawford, A. W., Michelsen, J. W. & Beckerle, M. C. J. Cell. Biol. 119, 1573–1587 (1992).
Zhadanov, A. B., Bertuzzi, S., Taira, M., Dawid, I. B. & Westphal, H. Dev. Dyn. 202, 354–364 (1995).
German, M. S., Wang, J., Chadwick, R. B. & Rutter, W. J. Genes Dev. 6, 2165–2176 (1992).
Bach, I. et al. Proc. Natl Acad. Sci. USA 92, 2720–2724 (1995).
Wadman, I. et al. EMBO J. 13, 4831–4839 (1994).
Karavanov, A. A., Saint-Jeannet, J.-P., Karavanova, I., Taira, M. & Dawid, I. B. Int. J. Dev. Biol. 40, 453–461 (1996).
Blanar, M. A. & Rutter, W. J. Science 256, 1014–1018 (1992).
Crawford, A. W., Michelsen, J. W. & Beckerle, M. C. J. Cell Biol. 116, 1381–1393 (1992).
Harper, J. W., Adami, G. R., Wei, N., Keyomarsi, K. & Elledge, S. J. Cell 75, 805–816 (1993).
Kintner, C. R. & Brockes, J. P. Nature 308, 67–69 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Agulnick, A., Taira, M., Breen, J. et al. Interactions of the LIM-___domain-binding factor Ldbl with LIM homeodomain proteins. Nature 384, 270–272 (1996). https://doi.org/10.1038/384270a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/384270a0
This article is cited by
-
Structural basis of the interaction between BCL9-Pygo and LDB-SSBP complexes in assembling the Wnt enhanceosome
Nature Communications (2023)
-
Aberrant DNA methylation in the PAX2 promoter is associated with Müllerian duct anomalies
Archives of Gynecology and Obstetrics (2020)
-
Interaction with ectopic cochlear crista sensory epithelium disrupts basal cochlear sensory epithelium development in Lmx1a mutant mice
Cell and Tissue Research (2020)
-
LHX2- and LDB1-mediated trans interactions regulate olfactory receptor choice
Nature (2019)
-
The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development
Scientific Reports (2018)
