Figure 4
From: Oncoprotein p28GANK binds to RelA and retains NF-κB in the cytoplasm through nuclear export
Molecular mechanisms of p28GANK alter RelA nuclear translocation. (A) Schematic representation of RelA (full) and its mutants, all fused with Flag-tag. Black boxes, nuclear localization signal (NLS); shaded boxes, Rel homology ___domain. (B) Interaction of overexpressed Myc-tagged p28GANK with Flag-tagged RelA mutants in vivo. HEK293 cells co-transfected with myc-tagged p28GANK and Flag-tagged deletion mutants of RelA (del-1 to del-4) were immunoprecipitated and immunoblotted using Myc or/and Flag antibody. (C) Schematic representation of p28GANK and its mutants with each of the ankyrin repeats (dA1 to dA5), the N terminus (dN) or the C terminus (dC) deleted, all fused with Myc-tag. (D) Interaction of overexpressed Flag-tagged RelA with Myc-tagged p28GANK mutants in vivo. HEK293 cells co-transfected with Flag-tagged RelA and Myc-tagged deletion mutants of p28GANK were immunoprecipitated and immunoblotted using Myc or/and Flag antibody.
