Extended Data Figure 6: PP1 RNAi cells differentiate less efficiently to procyclic forms than uninduced cells.
From: Genome-wide dissection of the quorum sensing signalling pathway in Trypanosoma brucei
a, Cells, induced or not to deplete PP1 by RNAi, collected on day 6 post infection and then incubated for 6 h in SDM79 culture medium in the presence of 6 mM cis-aconitate. The uninduced samples, which retain the ability to form stumpy cells, express procyclin whereas the induced cells, which remain slender, do not. The nucleus and kinetoplast DNA are stained with DAPI. Bar, 16 µm. b, Kinetoplast-posterior dimension of cells after 24 h in differentiation conditions. PP1 RNAi-induced cells (‘plus DOX’) have not repositioned their kinetoplast whereas PP1 uninduced cells (‘no DOX’) have undergone the normal kinetoplast repositioning associated with the differentiation of pleomorphic parasites to procyclic forms40. Horizontal bars represent the median distance in each population; y axis, microns. Example cells are shown below the graph; bar, 16 µm.
