Extended Data Figure 9: Transcripts regulated upon perturbed RBP7 expression in pleomorphic T. brucei.
From: Genome-wide dissection of the quorum sensing signalling pathway in Trypanosoma brucei
a, Scatterplots of the expression profile of genes from RBP7 RNAi (± DOX combined) cells versus RBP7B overexpressing cells induced with doxycycline. The full comparisons are available in Supplementary Data 4. The positions of several major transcripts up or downregulated in the respective comparisons are indicated to the left and right of each spot depending on whether they are more highly expressed in the RBP7 RNAi line or the overexpressing line, respectively. b, Expression profile of the top 20 genes that show opposing regulation upon RBP7 depletion by RNAi or overexpression. Reads map to both RBP7A and RBP7B such that they are not accurately distinguished in whole-transcriptome analysis, although specific analysis of these genes confirmed overexpression of RBP7B only (data not shown). Interestingly, similar specific analysis of PAD1 and PAD2 expression revealed that they were not detectably regulated by RBP7 overexpression or RNAi.
