Extended Data Figure 9: Phenotypes and confirmation of transgenic plants of D53-RNAi in d3 and d14.
From: DWARF 53 acts as a repressor of strigolactone signalling in rice
a, Phenotypes of wild-type, d3 and D53-RNAi/d3 transgenic plants. Scale bar, 10 cm. Four independent transgenic plants showed similar phenotypes. b, Phenotypes of wild-type, d14 and D53-RNAi/d14 transgenic plants at the mature stage. Scale bar, 10 cm. Five independent transgenic plants showed similar phenotypes. c, Tiller numbers of transgenic plants of D53-RNAi in d3 and d14 backgrounds at the mature stage. Values are means ± s.d. (n = 5). The double asterisks represent significant difference determined by the Student’s t-test at P < 0.01. d, The D53 abundance of D53-RNAi in d3 or d14 transgenic plants. Protein levels were analysed by immunoblotting using D53 polyclonal antibodies. Rice plants (T1 generation) were cultivated in the field in Hainan province. e, Sequence information of D53-RNAi constructs. Two complementary inverted D53 fragments are shown in black and the intron sequence between inverted DNA fragments is in red.
