Extended Data Figure 2: Cloning and confirmation of E9/D53.
From: DWARF 53 acts as a repressor of strigolactone signalling in rice
a, E9 was mapped in the interval between molecular markers Ds3 and K81114 on chromosome 11 using 142 recessive individual plants showing normal tillering phenotype from an F2 population. Numbers under the markers indicate recombinants. b, E9/D53 mutation sites in e9/d53 in the coding region and its amino acid changes. c, Schematic diagram of D53:d53 constructs. d, Phenotypes of wild-type and D53:d53 transgenic plants at the mature stage. Scale bar, 20 cm. Nine independent transgenic lines showed the similar tillering and dwarf phenotypes. e, Comparison of tiller numbers between wild-type and D53:d53 transgenic plants at the mature stage. Values are means ± s.d. (n = 15). The double asterisks represent significant difference determined by the Student’s t-test at P < 0.01. The plants were grown in the field in Beijing in the natural growing season.
