Extended Data Figure 5: Localization of CetZ1–GFP and variants, CetZ1–GFP(A206K) and CetZ1–mCherry, in H. volcanii. | Nature

Extended Data Figure 5: Localization of CetZ1–GFP and variants, CetZ1–GFP(A206K) and CetZ1–mCherry, in H. volcanii.

From: CetZ tubulin-like proteins control archaeal cell shape

Extended Data Figure 5

CetZ1 tagged with the GFP variant used primarily in this study, smRS-GFP40 was compared to CetZ1 tagged with the A206K mutant of smRS-GFP (which blocks GFP self-association51,52) and the mCherry protein51,53 (which shows red fluorescence in H. volcanii). Strains expressing the indicated CetZ1 fusion proteins in pTA962-based plasmids were sampled during mid-log phase growth in Hv-Ca + 0.2 mM Trp (log-phase cells) and from the leading edge of motile halos (0.2 mM Trp).

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