Extended Data Figure 5: β-Arrestin-mediated downstream signalling to kinases for M2-muscarinic, β2-adrenergic and FFA4 receptors.
From: β-Arrestin biosensors reveal a rapid, receptor-dependent activation/deactivation cycle
HEK293 cells were transfected with β2AR, M2-muscarinic or FFA4 receptors and stimulated with respective agonists at saturating concentrations (isoproterenol, 100 μM; carbachol (CCH), 100 μM; docosahexaenoic acid, 10 μM) for 10 min. β-Arrestin downstream signalling was evaluated by phospho-specific antibodies for pSrc, pERK1/2 and pJNK. Gβ was used as loading control. Data represent mean ± s.e.m. of n = 12 independent experiments. *P < 0.05 versus unstimulated control; #P < 0.05 versus indicated column (Kruskal–Wallis followed by Mann–Whitney U post-hoc analysis).
