Extended Data Figure 6: Concentration dependency of the kinetics of the conformational changes in β-arrestin upon β2AR stimulation.
From: β-Arrestin biosensors reveal a rapid, receptor-dependent activation/deactivation cycle
HEK293 cells were co-transfected with the β2AR and β-arrestin2–FlAsH2–CFP and stimulated with different concentrations of isoproterenol. Kinetics of the agonist evoked intramolecular FRET changes were analysed by curve fitting according to Fig. 2. The bar graph shows the rate constants τ (s) for conformational changes detected with the β-arrestin2–FlAsH2 sensor upon stimulation with 1, 10, 30 or 100 μM isoproterenol, respectively. Data represent mean ± s.e.m. of n ≥ 3 independent experiments. The values are not significantly different (P < 0.05).
