Extended Data Figure 5: Expression pattern and song phenotype of slo.
From: Natural courtship song variation caused by an intronic retroelement in an ion channel gene
a, Schematic of the generation of the sloLexA::P65 allele. T2A self-cleavage peptide sequences, LexA::P65, a 120 bp deletion, and a 3XP3::DsRed insertion were introduced into the first common exon shared by all slo transcripts in D. melanogaster. b, Schematic of the generation of the sloΔ2 allele. Two stop codons, a 120 bp deletion, and a 3XP3::DsRed insertion were introduced into the same locus indicated in panel a. The same allele was generated in each of the following D. simulans strains: sim5, sim202, sim203, sim205. PAM, protospacer adjacent motif. c, d, Brain (c) and ventral nerve cord (d) of D. melanogaster LexAop-nls::GFP, sloLexA::P65 males showed widespread expression of slo. e–h, Courtship song phenotype of sim5 sloΔ2 males. The slo-null males produced very little song (e). SinesPerMin and PulsesPerMin measure the average amount of sine song and pulse song produced per minute, respectively. The sine and pulse events of the slo-null males were severely disrupted: sine song waveform (f); pulse train (g); pulse song waveform (h). i, sloΔ2 heterozygotes sang sine song at 9.9 Hz lower frequency than wild-type. Data represent mean ± s.d. P values by one-way ANOVA.
