Extended Data Figure 6: Differential perturbation heat map view of the HDX studies.
From: Structure of the full-length glucagon class B G-protein-coupled receptor
a, Heat map view of the GCGR–NNC0640–mAb1 complex coloured according to the heat map colouring scheme used by the software HDX Workbench36. Each bar represents a peptide showing the average difference (across six time points) in D2O uptake between the receptor–antibody complex and the antibody-free receptor with the standard deviation between replicates and the peptide charge states shown in parentheses. The regions that revealed statistically significant reductions in deuterium uptake in the receptor–antibody complex compared to the antibody-free receptor are coloured green and boxed in red. The D2O difference between the antibody-bound and antibody-free GCGRs at two consecutive time points has a P value < 0.05 or a single time point has a P value < 0.01. The regions with no significant change are in grey and the regions that have no peptides covering the sequence in MSMS and HDX runs are shown as gaps. b, Heat map view of the GCGR–NNC0640–mAb23 complex.
