Extended Data Figure 10: Synapse loss, but not neuron or axon loss, is observed in the frontal cortex of 564Igi mice.

a, Staining for presynaptic (synaptophysin, red) and postsynaptic (homer, green) markers revealed structural synapses (colocalized markers, yellow). Scale bar, 25 μm. b, c, Significant decreases were seen in synaptophysin (b) and homer (c) puncta. n = 3 mice per group, *P < 0.05, **P < 0.01, unpaired t-test. d, Reduced synapse density was also observed in 8-week-old MRL-lpr mice by immunohistochemistry. n = 3 mice per group, *P < 0.05, unpaired t-test. e, Staining for the neuronal marker NeuN revealed similar neuronal density in the frontal cortex of 564Igi and wild-type mice. n = 3 mice per group, P = 0.85 (not significant), unpaired t-test. f, Staining for the axonal marker neurofilament-H showed a slight but not significant decrease in 564Igi axon density. n = 3 mice per group, P > 0.05 (not significant), unpaired t-test. Data are mean ± s.e.m. (b–f).