Extended Data Figure 3: Ifnb is not expressed and anti-IFNAR treatment is effective in both 564Igi and NZB/NZW mice.

a, qPCR for expression of Ifnb in the spleen showed similar expression levels in wild-type, 564Igi and NZB/NZW mice. n = 3 mice per group, P > 0.05, ANOVA. b, qPCR for expression of Ifnb in the hippocampus showed similar expression levels in wild-type, 564Igi and NZB/NZW mice. n = 3 mice per group, P > 0.05, ANOVA. c, qPCR for expression of Ifi44 in the spleen showed a significant increase in expression in 564Igi and NZB/NZW mice compared to wild-type mice that was reduced with anti-IFNAR treatment. n = 3 mice per group, *P < 0.049, **P = 0.002, two-way ANOVA with Sidak’s test. d, qPCR for expression of Oas1 in the spleen showed a significant increase in expression in 564Igi and NZB/W mice compared to wild-type mice that was reduced with anti-IFNAR treatment. n = 3 mice per group, *P = 0.03, ****P < 0.0001, two-way ANOVA with Tukey’s test. e, qPCR for expression of Ifi44 in the hippocampus showed a significant increase in expression in 564Igi and NZB/NZW mice compared to wild-type mice that was reduced with anti-IFNAR treatment. n = 3 mice per group, *P < 0.04, **P = 0.001, two-way ANOVA with Tukey’s test. f, qPCR for expression of Oas1 in the hippocampus showed a significant increase in expression in 564Igi and NZB/NZW mice compared to wild-type mice that was reduced with anti-IFNAR treatment. n = 3 mice per group, *P = 0.04, **P = 0.001, two-way ANOVA with Tukey’s test. g, qPCR for expression of Ifna in the spleen in 564Igi and NZB/NZW mice ± anti-IFNAR treatment. n = 3 mice per group, **P < 0.01, two-way ANOVA with Tukey’s test. h, Flow cytometry analysis of autoreactive B cell frequencies (percentage of B220⁺ cells) showed significant decreases after anti-IFNAR treatment. n = 4 mice per group, **P < 0.01, two-way ANOVA with Tukey’s test. i, qPCR for expression of Ifna in the hippocampus in 564Igi and NZB/NZW mice ± anti-IFNAR treatment. n = 3 mice per group, P > 0.05, two-way ANOVA with Tukey’s test. j, Representative image from a Mx1-Cre^Tdtomato reporter mouse immunostained against the neuronal marker NeuN (red), showing clusters of neurons and that are positive for Mx1 (pseudocoloured green). Scale bar, 50 μm. Data are mean ± s.e.m. (a–i).