Abstract
Three transgenic females from a first generation transgenic male were induced to lactate between 11 and 12 months of age using a series of estrogen and progesterone injections. The milk contained human longer acting tissue plasminogen activator (LAtPA) at comparable concentrations (1–3 mg/ml) as occurred in the original founder female. In addition, the transgenic male was induced with a hormonal regime and was shown to produce 0.85 mg/ml of LAtPA. Milk protein gels indicated that the milk products (casein, IgG) were essentially normal. These experiments show that expression data for this vector can be evaluated in a shorter period of time in dairy goats than would be required through normal gestation and lactation schedules and can be used to identify the relative expression of transgenes in mammary tissue that would occur during normal lactation.
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References
Ebert, K.M. and Schindler, J.E.S. 1993. Transgenic farm animals: Progress report. Theriogenology 39: 121–135.
Carver, A.S., Dalrymple, M.A., Wright, G., Cottom, D.S., Reeves, D.B., Gibson, Y.H., Keenan, J.L., Barrass, J.D., Scott, A.R., Colman, A. and Garner, I. 1993. Transgenic livestock as bioreactors: Stable expression of human alpha-1-antitrypsin by a flock of sheep. Bio/Technology 11: 1263–1270.
Lau, D., Kuzma, G., Wei, C.M., Livingston, D.J. and Hsiung, N. 1987. A modified human tissue plasminogen activator with extended half-life in vitro. Bio/Technology 5: 953–958.
Roberts, B., DiTullio, P., Vitale, J., Hehir, K. and Gordon, K. 1992. Cloning of the goat β-casein-encoding gene and expression in transgenic mice. Gene 255–262.
Ebert, K.M., Selgrath, J.P., DiTullio, P., Denman, J., Smith, T.E., Memon, M.A., Schindler, J.E., Monastersky, G.M., Vitale, J.A. and Gordon, K. 1991. Transgenic production of a variant of human tissue-type plasminogen activator in goat milk: Generation of transgenic goats and analysis of expression. Bio/Technology 9: 835–838.
Denman, J., Hayes, M., O'Day, C., Edmunds, T., Bartlett, C, Hirani, S., Ebert, K.M., Gordon, K. and McPherson, J.M. 1991. Transgenic expression of a variant of human tissue-type plasminogen activator in goat milk: Purification and characterization of the recombinant enzyme. Bio/Technology 9: 839–843.
Deharveng, G. and Nielsen, S.S. 1991. Partial purification and characterization of native plasminogen activators from bovine milk. J. Dairy Sci. 74: 2060–2072.
Alifakiotis, T.A., Katanos, I., Hatjiminaoglou, I., Zervas, N. and Zerfiridis, G. 1980. Induced lactation in diary ewes by various brief hormone treatments. J. Dairy Sci. 63: 750–755.
Thomas, P. 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77: 5201–5205.
Feinberg, A. and Vogelstein, B. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132: 6–13.
Laemmli, U. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.
Towbin, H., Staehelin, T. and Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76: 4350–4354.
Chomczynski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidiniun thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156–159.
Lehrach, H., Dramond, D., Wozney, J. and Boedtker, H. 1977. RNA molecular weight determination by gel electrophoresis under denaturing conditions: A critical reexamination. Biochemistry 16: 40–43.
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Ebert, K., DiTullio, P., Barry, C. et al. Induction of Human Tissue Plasminogen Activator in the Mammary Gland of Transgenic Goats. Nat Biotechnol 12, 699–702 (1994). https://doi.org/10.1038/nbt0794-699
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DOI: https://doi.org/10.1038/nbt0794-699
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