Figure 6: ANGPTL2 overexpression or deficiency alters AKT-SERCA2a signalling. | Nature Communications

Figure 6: ANGPTL2 overexpression or deficiency alters AKT-SERCA2a signalling.

From: ANGPTL2 activity in cardiac pathologies accelerates heart failure by perturbing cardiac function and energy metabolism

Figure 6

(a–d) Representative western blotting and quantitation of ANGPTL2, various signalling proteins and SERCA2a in hearts of Angptl2 KO (left), αMHC-Angptl2 Tg (right) and respective littermate mice. Hsc70 served as a loading control. n=3–6 per group. (e–h) Representative western blots and quantitation of indicated factors in NRCMs transfected with ANGPTL2 or control siRNA (left) or infected with Ad-Angptl2 or control Ad-LacZ (right). n=3–5 per group. Experiments were performed at least two times. (i) Left, representative western blot showing SERCA2a, AKT and ANGPTL2 protein levels in NRCMs transduced with Ad-LacZ+ siRNA control, Ad-LacZ+ siAngptl2 or Ad-dnAKT (dominant-negative AKT)+siAngptl2 (n=3). Hsc70 served as a loading control. Right, quantification of SERCA2a protein levels in blot at left. Levels in Ad-LacZ+ siRNA control were set to 1. Data are means±s.e.m. Control values were set to 1. Statistical significance was determined by Student’s t test (c,d,g and h) or one-way ANOVA (i). *P<0.05, **P<0.01 between genotypes or groups.

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