Figure 2: ALS2 gene editing using co-delivery of RNP complex and single-stranded (ss) oligo as repair template. | Nature Communications

Figure 2: ALS2 gene editing using co-delivery of RNP complex and single-stranded (ss) oligo as repair template.

From: Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

Figure 2

(a) Partial ALS2 sequence with ALS–gRNA target site, protospacer adjacent motif (PAM) is boxed and proline codon (amino acid position 165) to be edited is underlined. (b) Partial sequence of the ssDNA oligo used as a repair DNA template; modified nucleotides are shown in red font and serine codon is underlined. (c) Wild type (left) and ALS2 edited (right) plants tested for resistance to chlorsulfuron (200 mg l−1); shown at 10 days after spraying.

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