Table 2 Frequency of mutated alleles at intended and MS45 off-target sites in T0 plants regenerated after delivery of Cas9 and gRNAs as DNA vectors or RNP complexes.
From: Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
Target site | Cas9 and gRNA delivery method | Plants analysed | Plants with mutated alleles | Plants with biallelic mutations |
|---|---|---|---|---|
MS45 | DNA | 940 | 38 (4.0%) | 29 (3.1%) |
MS45 off-site | DNA | 940 | 19 (2.0%) | 4 (0.4%) |
MS45 | RNP | 1,880 | 70 (3.7%) | 9 (0.5%) |
MS45 off-site | RNP | 1,880 | 0 (0.0%) | 0 (0.0%) |
LIG | RNP | 756 | 73 (9.7%) | 7 (0.9%) |
MS26 | RNP | 756 | 18 (2.4%) | 2 (0.3%) |
