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QPrEST™—isotope-labeled multipeptide standards for quantitative mass spectrometry–based proteomics

Nature Methods volume 13, pages iv–vi (2016)Cite this article

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Abstract

Mass spectrometry (MS) enables absolute quantification of endogenous proteins by the use of isotope-labeled standards as internal references. QPrEST standards, currently available for >13,000 human proteins, represent a novel class of recombinantly produced heavy isotope–labeled standards that are added early in the quantification workflow. These multipeptide standards contain 50–150 amino acids identical to a human target sequence, resulting in absolute quantification data based on multiple tryptic peptides.

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Figure 1: Schematic of a QPrEST standard and its use.
Figure 2: Distribution of QPrEST products based on the number of unique tryptic peptides.
Figure 3: Absolute quantification of two human proteins using QPrEST standards.

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Authors and Affiliations

  1. Atlas Antibodies AB, AlbaNova University Center, Stockholm, Sweden

    Tove Boström

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  1. Tove Boström
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Correspondence to Tove Boström.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Boström, T. QPrEST™—isotope-labeled multipeptide standards for quantitative mass spectrometry–based proteomics. Nat Methods 13, iv–vi (2016). https://doi.org/10.1038/nmeth.f.393

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