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Hardware triggering: maximizing speed and efficiency for live cell imaging

Abstract

Live cell imaging experiments now require higher speeds and more data throughput than ever before. Nikon Instruments has robust tools that enable hardware triggering of imaging devices in microscopy via direct signaling between hardware. This minimizes delays, synchronizes devices, and reduces the exposure of specimens to light. This Application Note explains how Nikon's NIS-Elements hardware-triggering workflow operates, and details its benefits for common time-lapse acquisition routines.

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Figure 1: Exposure signal times the movement of other devices via direct hardware triggers.
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Correspondence to Michael A Davis.

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Davis, M. Hardware triggering: maximizing speed and efficiency for live cell imaging. Nat Methods 14, 1223 (2017). https://doi.org/10.1038/nmeth.f.403

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