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Improving biosensor assay development by determining sample quality with Tycho NT.6

Nature Methods volume 15, page 298 (2018)Cite this article

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Abstract

Surface plasmon resonance (SPR) is an optical methodology widely used to detect and quantify molecular interactions. SPR is considered the gold standard for quantification of protein interactions, but SPR assay optimization can be technically challenging, time-consuming and costly. Tycho™ NT.6 quickly analyzes different conditions typically tested during optimization of an SPR assay. The Tycho™ NT.6 system is simple to use and enables researchers to make better-educated decisions in developing and optimizing their binding-interaction assays.

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Figure 1: Lower-pH buffer conditions are better for immobilization of ligand to the SPR biosensor chip, as determined by pH scouting experiments.
Figure 2: Quick evaluation of immobilization buffer conditions with Tycho NT.6.

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  1. NanoTemper Technologies GmbH, Munich, Germany

    Dennis Breitsprecher, Peter A Fung & Nuska Tschammer

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  1. Dennis Breitsprecher
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  2. Peter A Fung
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  3. Nuska Tschammer
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Correspondence to Peter A Fung.

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Breitsprecher, D., Fung, P. & Tschammer, N. Improving biosensor assay development by determining sample quality with Tycho NT.6. Nat Methods 15, 298 (2018). https://doi.org/10.1038/nmeth.f.406

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