Abstract
*Purpose:* To investigate the MR imaging of Bifidobacterium longum and Clostridium novyi-NT labeling with superparamagnetic iron oxide (SPIO) nanoparticles.
*Materials and methods:*
Tubes containing B. longum-SPIO, Free-SPIO, B. longum and PYG Medium were incubated under anaerobic condition in in vitro experiment. Transmission electron microscope and Prussian blue staining were used to demonstrate intra-bacteria nanoparticles. R~2~^*^ mapping and R~2~ mapping were reconstructed after MR scanning. B. longum-SPIO and C. novyi-NT-SPIO were injected respectively in vivo to show whether it might be traced by MR imaging.
*Results:*
Magnetosomes in bacteria were observed by electron microscopic and stained by Prussian blue staining. At the same concentration of SPIOs, the R~2~^*^ value of B. longum-SPIO was significantly higher than that of Free-SPIO (P<0.001), however, the R~2~ value was lower comparing with Free-SPIO (P<0.001). After injection with B. longum-SPIO, they could present in tumor and shorten T~2~^*^.*Conclusion:* B. longum and C. novyi-NT could be labeled by SPIO and then traced by MRI.
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Wang, J., Zhang, X., Zhou, D. et al. Magnetic Resonance imaging (MRI) in detection of Bifidobacterium longum and Clostridium novyi-NT labeled with superparamagnetic iron oxide (SPIO) nanoparticle. Nat Prec (2009). https://doi.org/10.1038/npre.2009.3435.1
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DOI: https://doi.org/10.1038/npre.2009.3435.1
