Supplementary Figure 8: Detection of functional sRNA by TEsR—the rare telomeric DNA damage response RNA (tDDRNA).
From: Target-enrichment sequencing for detailed characterization of small RNAs
(a) TEsR generated deep sequencing information for the rare telomeric DNA damage response RNA (tDDRNA) at thousands of reads. To avoid spurious reads contamination, only reads that had exact sequence match to the expected telomeric repeats (TAAGGG) were counted, and telomeric reads with mismatches or soft clipped portions were removed with ad hoc R scripts. The majority of reads were from 18-22nt in size. (b) In contrast, tDDRNAs were hardly detected (4 reads over a total of 207 million reads) by deep HiSeq sequencing of small RNA samples or total RNA samples.
References
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