Fig. 6: Downregulation of MED16 reduces S6 phosphorylation.
From: A brain somatic RHEB doublet mutation causes focal cortical dysplasia type II
HEK293T cells were transfected with scrambled control siRNA (NC) or MED16 siRNAs (siRNA1, siRNA2, and siRNA3) for 48 h. a The relative MED16 mRNA levels in transfected cells were quantified by quantitative real-time PCR and normalized to the β-actin mRNA levels for comparison. b Cell lysates were analyzed by SDS-PAGE and immunoblotting to detect phosphorylated S6 (pS6), total S6 and α-tubulin. c The levels of pS6 were normalized to those of total S6 for comparison. d The levels of S6 were normalized to those of α-tubulin for comparison. Data are presented as the mean ± s.e.m, n = 4, *P < 0.05, **P < 0.01, ***P < 0.001, ns: not significant (Student’s t-test)
