Fig. 1: IgSF11 expression is quickly upregulated in vitro, differentiating osteoclasts and osteoarthritic subchondral bone.
From: IgSF11 deficiency alleviates osteoarthritis in mice by suppressing early subchondral bone changes
a BMMs from WT and IgSF11−/− mice were induced to differentiate into mature osteoclasts by culture with M-CSF and RANKL for 3 days. The protein levels of IgSF11, the osteoclast activation-related protein NFATc1, and β-actin at 1, 2, and 3 days were detected by western blotting. b–d WT and IgSF11−/− mice underwent DMM or sham surgery. b The subchondral bone was harvested at 1 or 2 weeks and subjected to qRT‒PCR analysis of IgSF11. c, d Whole joint sections were subjected to immunofluorescence analysis of IgSF11 (green) at 1 week. c Representative immunofluorescence images. AC articular cartilage, SBP subchondral bone plate, STB subchondral trabecular bone. Scale bars, 100 μm. d shows the IgSF11+ cells per bone marrow area (mm2). Error bars show the S.E.M. for n = 4/strain. Two-way ANOVA followed by Tukey’s multiple comparison test was conducted. p values are indicated in the figures (***p < 0.001).
