Fig. 4 | International Journal of Oral Science

Fig. 4

From: USP34 regulates tooth root morphogenesis by stabilizing NFIC

Fig. 4

USP34 deubiquitinates and stabilizes NFIC. a Co-immunoprecipitation of USP34 with ectopically expressed NFIC in 293 T cells. b Loss of USP34 increased the degradation rate of NFIC as determined by CHX chase assay. 293 T cells were starved overnight, treated with CHX and harvested at indicated time periods. c Immunoblot of NFIC-linked polyubiquitin. 293 T cells were transfected with siUSP34, HA-Ub, and then treated with 10 μM MG132 for 4 h. d Quantification of NFIC degradation rate

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