Fig. 6
From: Sema3A secreted by sensory nerve induces bone formation under mechanical loads
Sema3A maintains the spreading morphology of hPDLCs under mechanical overloads via the ROCK2. a Phalloidin staining showed that mechanical overload stretched hPDLCs, and Sema3A can maintain the spreading morphology of hPDLCs. b Western Blot showed that mechanical overload inhibited the expression of ROCK2 protein in hPDLCs, and exogenous Sema3A restored ROCK2 protein expression. c, d Quantification of the aspect ratio (c) and circularity (d) of hPDCLs in (a) (n = 5). e Quantification of ROCK2 protein in (b) (n = 3). f Phalloidin staining showed that the ROCK2 inhibitor Y27632 blocked the effect of Sema3A on F-actin spreading. g Immunofluorescence showed that Y27632 inhibited the efforts of Sema3A on the expression of Osx under mechanical overload. h, i Quantification of the aspect ratio (h) and circularity (i) of hPDLCs in (f) (n = 5). j Quantification of Osx in (g) (n = 5). All the quantitative data in Fig. 6 is presented as mean ± SD, and Two-tailed Student’s t-test was used for comparison. *P < 0.05; **P < 0.01; ***P < 0.001
