Fig. 6 | International Journal of Oral Science

Fig. 6

From: Integrative single-cell and bulk transcriptomes analyses reveals heterogeneity of serine-glycine-one-carbon metabolism with distinct prognoses and therapeutic vulnerabilities in HNSCC

Fig. 6

IMPDH inhibitor represses HNSCC cell progression in vitro. a The decentralized bar chart shows the differentially expressed metabolic pathways based on ssGSEA analysis between high- and low-risk groups in TCGA-HNSC dataset. b Violin plot of IMPDH1 expressions in high- and low-risk groups. c CCK8 was applied to test the sensitivities of HNSCC cells to different doses of MPA or MMF. The IC50s were calculated. After treating with DMSO or MPA (10 μmol/L), the cell viability of HNSCC cells were determined using CCK-8 (d), the proliferation rates were examined by EdU assay (e), the cell cycle (f) and apoptosis (g) were tested using flow cytometry, the protein levels of endogenous apoptosis markers were measured by western blot (h), the cell migration ablility was determined by Would healing assay (i) and Transwell assay without matrigel (j), and the invasion ability was detected by Transwell assay with matrigel (k). Mean ± s.d.; *P < 0.05; (d) two-way ANOVA, (e−g, i−k) Student’s t tests. Scale bars, (e) 50 μm, (i) 100 μm, (j, k) 200 μm

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